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Ultrafiltration is an important step to separate, purify and concentrate protein solutions in biopharmaceutical manufacturing, improving production capacity and recovery rate. The retention capacity of ultrafiltration membranes are characterized by the molecular weight cut-off (MWCO) of the target protein, typically ranging from 1kDa to 1000kDa.
Determine the MWCO of Ultrafiltration Membrane
- To have the target macromolecule permeate the membrane, generally choose an ultrafiltration membrane with a MWCO that is 3-5 times bigger than the MW of target macromolecule.
- To have the target macromolecule to be retained, generally choose an ultrafiltration membrane with a MWCO that is 3-5 times smaller than the MW of target macromolecule.
Choose the Right TFF Cassettes
| Polyethersulfone（PES） | Regenerated Cellulose（RC） | |
| Features | pH range: 1-14 High flux, very low protein binding |
Natural Hydrophilic Resistant to organic solvents Suitable for high concentration formulation |
| MWCO | 1, 3, 5, 8, 10, 30, 50, 100, 300, 500, 1000 kDa | 1, 2, 3, 5, 8, 10, 30, 50, 100, 300 kDa |
| Typical Applications | Oligonucleotide, Peptide, Proteins, Growth factor, FVIII，Recombinant proteins，mAb, Vaccines, Viral vectors | Peptide, Insulin, Growth factor, Recombinant proteins，mAb, Collagen, LNP, Exosome, Viral vectors |
| Filtration Area | 100cm2, 200 cm2, 0.11 m2, 0.46m2, 0.5 m2, 2.33 m2, 2.5 m2 | |
Choose the Right Hollow Fiber TFF
| Fiber ID | Effective Length | Batch Volumes | Feed/Retentate Connectors | Permeate Connectors | MWCO | |
| Mini | 0.5mm 1.0mm |
30cm 60cm |
<600mL | Female Luer | Female Luer | 3 kDa 5 kDa 10 kDa 30 kDa 50 kDa 100 kDa 300 kDa 500 kDa 750 kDa |
| Minilab | 0.5mm 1.0mm |
30cm 60cm |
<2L | Female Luer | Female Luer | |
| Lab | 0.5mm 1.0mm |
30cm 60cm |
2L-16L | 0.5'' TC | 3/16'' HB | |
| Pilot | 0.5mm 1.0mm |
30cm 60cm 110cm |
15L-80L | 1.5'' TC | 0.5'' TC | |
| Process | 0.5mm 1.0mm |
30cm 60cm 110cm |
75L-800L | 1.5'' TC | 1.0'' TC | |
| Maxi | 0.5mm 1.0mm |
30cm 60cm 110cm |
800L-1500L | 2.0'' TC | 1.5'' TC |
